1, 10-PHENANTHROLINE (PL) IS THE PARENT OF AN IMPORTANT CLASS OF CHELATING AGENTS THAT HAS EXCELLENT PROPERTIES; THE PLANAR NATURE OF 1, L0-PHENANTHROLINE IS ITS ABILITY TO PARTICIPATE AS EITHER AN INTERCALATING OR GROOVE-BINDING SPECIES WITH DNA AND RNA AND THE RIGID PL MOLECULE DEMONSTRATED DISTINCT EFFECTS ON IN VITRO CULTURED CELLS [1]. THEREFORE DETERMINATION OF THIS COMPLEX AND PL SINGLY, IN BIOLOGICAL SAMPLES IS VERY IMPORTANT. WE USED PHOTOTHERMAL SPECTROMETRY FOR DETERMINATION OF TRACE AMOUNT OF SAMARIUM COMPLEX AND PL IN A MICROCHIP.PHOTOTHERMAL SPECTROMETRY IS A CLASS OF HIGHLY SENSITIVE METHODS BASED ON MEASUREMENT OF AMOUNT OF THERMAL ENERGY RELEASED BY A SAMPLE SUBSEQUENTLY TO LIGHT ABSORPTION AND NONRADIATIVE RELAXATION OF THE EXITED SPECIES. WHEN A SAMPLE IS IRRADIATED BY A LASER BEAM, LOCAL HEATING NEAR THE BEAM AXIS PRODUCES A TRANSVERSE TEMPERATURE GRADIENT WHICH SUBSEQUENTLY INDUCES THE FORMATION OF A REFRACTIVE INDEX GRADIENT. MOST GENERALLY, THE REFRACTIVE INDEX IS LOWERED BY THE TEMPERATURE RISE SO THAT THE REFRACTIVE INDEX GRADIENT IS NEGATIVE AND BEHAVES LIKE A DIVERGING LENS. PRACTICALLY, ONE LASER IS USED TO CREATE PHOTOTHERMAL EFFECT AND A SECOND LASER IS USED TO MONITOR THE THERMAL LENS [2]. THERMO OPTICAL SPECTROSCOPY IS AMONG THE METHODS THAT HAVE ALREADY PROVEN THEIR EXPEDIENCE IN COMBINING WITH CHEMICAL MICROCHIP, AS WELL AS FOR OTHER MICROSCALE MEASUREMENTS. THERMAL LENS MICROSCOPY SHOWED MUCH POTENTIAL WHEN DEALING WITH ULTRATRACE ON-CHIP DETERMINATION OF VARIOUS SUBSTANCES AND THE ANALYSIS OF BIOLOGICAL MATERIALS [3].

IN THIS RESEARCH, WE HAVE CONSTRUCTED AND DEVELOPED A PHOTOTHERMAL LENS MICROSCOPEMICROCHIP ELECTROPHORESIS SYSTEM (PTLM-MCE) AS A HIGHLY SENSITIVE SEPARATION SYSTEM.LAB ON CHIP PRESENTS AS TOP INSTRUMENTAL FIELD IN ANALYTICAL CHEMISTRY. MINIATURIZATION, INTEGRATION AND AUTOMATION ARE GOALS OF MICRO TOLAL ANALYSIS SYSTEMS. ...

IN THIS WORK, MICROCHIP ELECTROPHORESIS (ME) COMBINED WITH AN INVERT THERMAL LENS MICROSCOPE (TLM) AS DETECTOR WAS SUCCESSFULLY APPLIED FOR THE ANALYSIS OF TRACE MEASUREMENTS OF METAL ION COMPLEXES. ...

SENSITIVITY, THERMOOPTICAL PROPERTIES AND LOW SAMPLE CONSUMPTION ARE MAJOR PROPERTIES THAT HIGHLIGHTED PHOTO THERMAL LENS MICROSCOPY (PTLM) FOR DETECTION OF SAMPLES. IN THIS WORK, A COMBINED FLOW INJECTION PHOTOTHERMAL LENS MICROSCOPY WAS UTILIZED FOR HIGHLY SENSITIVE DETERMINATION OF METHIMAZOLE AS AN ANTI-HYPERTHYROID DRUG IN PHARMACEUTICAL SAMPLES BY USING GOLD NANO PARTICLES (GNPS)...

SENSITIVE DETECTION OF NONFLUORESCENT MOLECULES AT THE FEMTOLITER (FL) SCALE HAS BEEN ACHIEVED IN A MICRO FLUIDIC CELL. SMALLER SAMPLE VOLUMES AND HIGHER SENSITIVITY HAVE BEEN SIGNIFICANT TARGETS FOR USING PHOTOTHERMAL LENS MICROSCOPY AS A DETECTION SYSTEM...

PHOTOTHERMAL SPECTROSCOPY IS A GROUP OF HIGH SENSITIVITY SPECTROSCOPY TECHNIQUES USED TO MEASURE OPTICAL ABSORPTION AND THERMAL CHARACTERISTICS OF A SAMPLE. THE BASIS OF PHOTOTHERMAL SPECTROSCOPY IS THE CHANGE IN THERMAL STATE OF THE SAMPLE RESULTING FROM THE ABSORPTION OF RADIATION [1]. PLASMONIC NANOPARTICLES (NP) ARE THE BEST KNOWN CONVERTERS OF OPTICAL ENERGY INTO HEAT DUE TO THEIR UNIQUE MECHANISM OF PLASMON RESONANCE. IN THIS WORK WE USED OF A SINGLE BEAM THERMAL LENS APPARATUS UNDER DIOD LASER EXCITATION (532 NM). IN ORDER TO INVESTIGATE THE INTERACTION OF GOLD NANOPARTICLES WITHMACROMOLECULES THAT HAVE ELECTRONEGATIVE GROUPS, WE USED THIACALIX [4] AREN DERIVED (1)1 AND THIACALIX [4] AREN DERIVED (2) 2, THE STRUCTURE OF THIS MACROCYCLES SHOWS IN FIG (1) AND FIG (2) RESPECTIVELY.